You don't want to stare at it looking for the dot.....you want to look away then glance at the tube quickly. Do you see it?
Also its best to perform this test outside in the bright sunlight. Keep the sun at your back, holding the vial waist high.....then do the test. If unsure you can pour it back into the test bottle again and try again. I like to actually fill to each successive line directly and then do the glance thing.
Maddie