Ok- I think things have become a bit confused around the CYA test here- if you initially reported a CYA level of 100 or greater, then did the 50:50 dilution x2 you should expect the black dot to disappear before you fill the CYA tube. In other words If you are greater than 100 you would expect the black dot to disappear.
Perhaps go back and perform the 50:50 test. Have you checked that you are conducting this correctly?
Perhaps go back and perform the 50:50 test. Have you checked that you are conducting this correctly?